phospho-FOXO4 (Thr451) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13208R-Cy7-100uL |
| Product Name | phospho-FOXO4 (Thr451) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FOXO4 around the phosphorylation site of Thr451 |
| Modification Site | Thr451 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4303 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | FOXO4 phospho T451;p-FOXO4 phospho T451; AFXSer197; Fork head domain transcription factor AFX1; AFX; AFX1; Afxh; ALL1-fused gene from X chromosome; Fork head domain transcription factor AFX1; Forkhead box O4; Forkhead box protein O4; FOXO 4; Mixed lineage leukemia, translocated to, 7; MLLT7; Myeloid lymphoid or mixed lineage leukemia translocated to 7; Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7; Putative fork head domain transcription factor AFX1; FOXO4_HUMAN. |
| Background | FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-FOXO4 (Thr451) Polyclonal Antibody, Cy7 Conjugated (bs-13208R-Cy7) validated for, and at what dilution?
bs-13208R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-FOXO4 (Thr451) Polyclonal Antibody, Cy7 Conjugated (bs-13208R-Cy7) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Pig, Rabbit.
How should bs-13208R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-FOXO4 (Thr451) Polyclonal Antibody, Cy7 Conjugated (bs-13208R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human FOXO4 around the phosphorylation site of Thr451
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13208R-Cy7)?
Cy7 on bs-13208R-Cy7: excitation / emission 743nm/767nm.