Frizzled 8 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13219R-BF405 |
| Product Name | Frizzled 8 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Frizzled 8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8325 |
| Subcellular location | Extracellular |
| Synonyms | Frizzled 8 seven transmembrane spanning receptor; frizzled 8, seven transmembrane spanning receptor; Frizzled family receptor 8; frizzled homolog 8 Drosophila; Frizzled homolog 8; Frizzled-8; FZ 8; Fz-8; FZ8; FZD 8; FZD8; FZD8_HUMAN; hFZ 8; hFZ8; HGNC4046; Homolog of Drosophila Frizzled 8. |
| Background | The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled, as well as several secreted mammalian frizzled-related proteins (FRPs), have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy-terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. The cysteine-rich domain of frizzled-8 blocks endogenous Wnts and the effects of Wnt-1 and Wnt-5 on proliferation. The mouse frizzled-8 gene, which encodes a Wnt receptor, is a potent cancer-associated activator of the Beta-catenin-TCF pathway. The frizzled-8 gene contains no introns. Frizzled-8 mRNA has been detected in fetal brain and kidney, and also in adult pancreas, skeletal muscle, kidney and heart. Frizzled is highly expressed in HeLa S3 (cervical uterus cancer) cells and A549 lung cancer cells. |
| Application Dilution | |
| WB | |
| IF(IHC-P) | |
Frequently asked questions
Which applications is Frizzled 8 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-13219R-BF405) validated for, and at what dilution?
bs-13219R-BF405 is validated for WB, IF(IHC-P).
Which species does Frizzled 8 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-13219R-BF405) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow.
How should bs-13219R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Frizzled 8 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-13219R-BF405)?
KLH conjugated synthetic peptide derived from human Frizzled 8