FZD3 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.$

Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

$Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 overnight at 4\u00b0C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 overnight at 4\u00b0C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tRabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated ( \\tbs-13239R ) at 1:200 overnight at 4\u00b0C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy)and used at 1:200 dilution for 40 minutes at 37\u00b0CDAPI(5ug\/ml) was used to stain the cell nuclei$

Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tRabbit Anti-FZD3\/Frizzled 3 Polyclonal Antibody, Unconjugated ( \\tbs-13239R ) at 1:200 overnight at 4\u00b0C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy)and used at 1:200 dilution for 40 minutes at 37\u00b0CDAPI(5ug\/ml) was used to stain the cell nuclei

$Lane 1: Human SW480 cell Lysates; Lane 2: Human U-2 OS cell Lysates; Lane 3: Human A431 cell Lysates; Lane 4: Human SH-SY5Y cell Lysates. Probed with FZD3 polyclonal Antibody, unconjugated (bs-13239R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human SW480 cell Lysates; Lane 2: Human U-2 OS cell Lysates; Lane 3: Human A431 cell Lysates; Lane 4: Human SH-SY5Y cell Lysates. Probed with FZD3 polyclonal Antibody, unconjugated (bs-13239R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA

Reactivity

Human
Rat

Overview
Catalog #	bs-13239r
Product Name	FZD3 Polyclonal Antibody
Applications	WB ELISA
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human FZD3 protein
Immunogen Range	121-220/666
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7976
Swiss Prot	Q9NPG1
Subcellular location	Cell membrane
Synonyms	Fz-3; D930050A07Rik; Fz3; mFz3; FZD3_HUMAN; FZD3; hFz3; FZD3_MOUSE; frizzled class receptor 3; frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); frizzled 3, seven transmembrane spanning receptor; frizzled family receptor 3; Frizzled-3
Background	Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Application Dilution
WB	1:500-1000
ELISA	1:5000-10000