FZR1/CDC20C Polyclonal Antibody

$Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-FZR1\/CDC20C Polyclonal Antibody, Unconjugated (bs-13240R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-FZR1\/CDC20C Polyclonal Antibody, Unconjugated (bs-13240R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Mouse intestine lysates probed with Rabbit Anti-FZR1\/CDC20C Polyclonal Antibody, Unconjugated (bs-13240R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.$

Mouse intestine lysates probed with Rabbit Anti-FZR1\/CDC20C Polyclonal Antibody, Unconjugated (bs-13240R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Mouse testis lysates probed with Rabbit Anti-FZR1\/CDC20C Polyclonal Antibody, Unconjugated (bs-13240R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.$

Mouse testis lysates probed with Rabbit Anti-FZR1\/CDC20C Polyclonal Antibody, Unconjugated (bs-13240R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

$Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Rat Liver tissue lysates ; Lane 5: Rat Testis tissue lysates; Lane 6: Human U251 cell lysates; Lane 7: Human A431 cell lysates probed with FZR1\/CDC20C Polyclonal Antibody, Unconjugated (bs-13240R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Rat Liver tissue lysates ; Lane 5: Rat Testis tissue lysates; Lane 6: Human U251 cell lysates; Lane 7: Human A431 cell lysates probed with FZR1\/CDC20C Polyclonal Antibody, Unconjugated (bs-13240R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-13240R
Product Name	FZR1/CDC20C Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FZR1/CDC20C
Immunogen Range	141-240/496
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51343
Swiss Prot	Q9UM11
Subcellular location	Cytoplasm, Nucleus
Synonyms	FZR; FZR2; CDC20C; Fizzy-related protein homolog; CDC20-like protein 1; Cdh1/Hct1 homolog; FZR1; FYR; KIAA1242
Background	Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500