G gamma14 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13243R-Cy5 |
| Product Name | G gamma14 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human G gamma14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | G GAMMA 8; G gamma 9; G GAMMA C; G protein cone gamma 8 subunit; gamma T2 subunit; GNG8; GNG9; GNGT2; GNGT8; guanine nucleotide binding protein G protein, gamma transducing; Guanine nucleotide binding protein G; guanine nucleotide binding protein gamma 9; guanine nucleotide binding protein gamma transducing activity; IG; Ogamma T2 subunit precursor; SG; GBGT2_HUMAN. |
| Background | G Gamma14 (GNGT2) is a Guanine nucleotide-binding protein. G proteins are composed of 3 units, alpha, beta and gamma and are involved in various transmembrane signaling systems as signal modulators or transducers. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. This G protein is specific to retinal cones and is involved in regulation of phototransduction. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is G gamma14 Polyclonal Antibody, Cy5 Conjugated (bs-13243R-Cy5) validated for, and at what dilution?
bs-13243R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13243R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise G gamma14 Polyclonal Antibody, Cy5 Conjugated (bs-13243R-Cy5)?
KLH conjugated synthetic peptide derived from human G gamma14
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13243R-Cy5)?
Cy5 on bs-13243R-Cy5: excitation / emission 625,650nm/670nm.