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phospho-GABBR2 (Ser884) Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-13258R-PerCP-Cy5.5-100uL
Product Name phospho-GABBR2 (Ser884) Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation PerCP-Cy5.5
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human GABBR2 around the phosphorylation site of Ser884
Modification Site Ser884
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9568
Subcellular location Cell membrane
Synonyms GABA B Receptor 2 phospho S884; p-GABA B Receptor 2 phospho S884; p-GABBR2 Ser884 ; GABBR2; GB2; G protein coupled receptor 51; G-protein coupled receptor 51; GAB B R2; GABA B R2; GABA B receptor 2; GABA B RECEPTOR; GABA-B receptor 2; GABA-B-R2; GABA-BR2; GABA[[B]]R2; GABAB R2; GABABR 2; GABABR2; GABB R2; GABBR 2; Gabbr2; GABR2_HUMAN; Gamma aminobutyric acid GABA B receptor 2; Gamma aminobutyric acid B receptor 2; Gamma aminobutyric acid GABA B receptor 2; Gamma aminobutyric acid type B receptor subunit 2; Gamma-aminobutyric acid type B receptor subunit 2; Gb 2; Gb2; GH07312; GPR 51; GPR51; GPRC 3B; GPRC3B; HG 20; HG20; HRIHFB2099; Metabotropic GABA B receptor subtype 2; OTTHUMP00000021776; OTTHUMP00000063797; R2 SUBUNIT; BcDNA:GH07312; CG6706; CT20836; D GABA[[B]]R2; D Gaba2; FLJ36928.
Background The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is phospho-GABBR2 (Ser884) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-13258R-PERCP-CY5-5) validated for, and at what dilution?

BS-13258R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-GABBR2 (Ser884) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-13258R-PERCP-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig.

How should BS-13258R-PERCP-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-GABBR2 (Ser884) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-13258R-PERCP-CY5-5)?

KLH conjugated synthetic phosphopeptide derived from human GABBR2 around the phosphorylation site of Ser884

What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-13258R-PERCP-CY5-5)?

PerCP-Cy5.5 on BS-13258R-PERCP-CY5-5: excitation / emission 482nm/694nm.

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