GALM Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13268R-Cy7 |
| Product Name | GALM Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GALM/Galactose mutarotase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 130589 |
| Subcellular location | Cytoplasm |
| Synonyms | Aldose 1 epimerase; Aldose 1-epimerase; BLOCK25; Galactomutarotase; Galactose mutarotase; Galm; GALM_HUMAN; IBD1. |
| Background | Galactose Mutarotase is a member of the aldose epimerase family and is involved in hexose metabolism. Through its catalytic activity, Galactose Mutarotase converts beta-aldose to alpha-aldose on several sugars, including D-glucose, L-arabinose and D-xylose. Found in the cytoplasm of most cells, Galactose Mutarotase plays a key role in galactose metabolism by catalyzing the conversion of beta-D-galactose to alpha-D-galactose. The enzyme contains two residues, Glu 304 and His 170, that are critical for catalysis, as well as His 96 and Asp 243, which are important for proper substrate recognition by the active site. No known diseases have been associated with mutations in the Galactose Mutarotase gene, although inhibition of Galactose Mutarotase activity could potentially be associated with a build-up of unmetabolized sugars during metabolism. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GALM Polyclonal Antibody, Cy7 Conjugated (bs-13268R-Cy7) validated for, and at what dilution?
bs-13268R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GALM Polyclonal Antibody, Cy7 Conjugated (bs-13268R-Cy7) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse.
How should bs-13268R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GALM Polyclonal Antibody, Cy7 Conjugated (bs-13268R-Cy7)?
KLH conjugated synthetic peptide derived from human GALM/Galactose mutarotase
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13268R-Cy7)?
Cy7 on bs-13268R-Cy7: excitation / emission 743nm/767nm.
