GALNT11/GalNAc-T11 Polyclonal Antibody

$Raji Cell lysates probed with GALNT11\/GalNAc-T11 Polyclonal Antibody, unconjugated (bs-13271R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Raji Cell lysates probed with GALNT11\/GalNAc-T11 Polyclonal Antibody, unconjugated (bs-13271R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Siha Cell lysates probed with GALNT11\/GalNAc-T11 Polyclonal Antibody, unconjugated (bs-13271R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Siha Cell lysates probed with GALNT11\/GalNAc-T11 Polyclonal Antibody, unconjugated (bs-13271R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Horse
Rabbit

Overview
Catalog #	bs-13271R
Product Name	GALNT11/GalNAc-T11 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GALNT11/GalNAc-T11
Immunogen Range	401-500/608
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	63917
Subcellular location	Cytoplasm, Cell membrane
Synonyms	A430075I06Rik; AI648252; E430002F06Rik; FLJ21634; GalNAc-T11; GALNT11; GLT11_HUMAN; MGC71630; Polypeptide GalNAc transferase 11; Polypeptide N-acetylgalactosaminyltransferase 11; pp-GaNTase 11; Protein-UDP acetylgalactosaminyltransferase 11; tcag7.1057; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11.
Background	The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosamine) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in the Golgi apparatus. GalNAc-T11 (Polypeptide N-acetylgalactosaminyltransferase 11), also known as UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11, is a 608 amino acid protein that catalyzes glycosylation of Muc1, Muc4.1 and EA2, though it does not display enzymatic preference for erythropoitein. The N-terminal domain is involved in substrate binding and manganese coordination, while the C-terminal domain is involved in UDP-Gal binding and catalytic reaction. GalNAc-T11 is highly expressed in kidney tubules, though it is not expressed in glomeruli. There are two isoforms of GalNAc-T11 that are produced as a result of alternative splicing events.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500