Tenascin C Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1327R-BF647-100uL |
| Product Name | Tenascin C Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tenascin C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3371 |
| Swiss Prot | P24821 |
| Subcellular location | Extracellular matrix |
| Synonyms | Cytotactin; Glioma associated extracellular matrix antigen; GMEM; GP 150 225; Hexabrachion; HXB; JI; Myotendinous antigen; Neuronectin; Tenascin C; TenascinC; TN; TN C; TNC.Tn-C; Tenascin-C. |
| Background | Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Tenascin C Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1327R-BF647) validated for, and at what dilution?
bs-1327R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tenascin C Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1327R-BF647) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Rabbit.
How should bs-1327R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tenascin C Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1327R-BF647)?
KLH conjugated synthetic peptide derived from human Tenascin C
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-1327R-BF647)?
AbBy Fluor-647 on bs-1327R-BF647: excitation / emission 650nm/665nm.