GBA2 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13297R-Cy7 |
| Product Name | GBA2 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GBA2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57704 |
| Swiss Prot | Q9HCG7 |
| Subcellular location | Cell membrane |
| Synonyms | AD035; SPG46; NLGase; Non-lysosomal glucosylceramidase; Beta-glucocerebrosidase 2; Beta-glucosidase 2; Glucosylceramidase 2; GBA2; KIAA1605 |
| Background | Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide (GlcCer) to free glucose and ceramide. Involved in sphingomyelin generation and prevention of glycolipid accumulation. May also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo. Plays a role in central nevous system development. Required for proper formation of motor neuron axons. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GBA2 Polyclonal Antibody, Cy7 Conjugated (bs-13297R-Cy7) validated for, and at what dilution?
bs-13297R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GBA2 Polyclonal Antibody, Cy7 Conjugated (bs-13297R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Chicken.
How should bs-13297R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GBA2 Polyclonal Antibody, Cy7 Conjugated (bs-13297R-Cy7)?
KLH conjugated synthetic peptide derived from human GBA2
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13297R-Cy7)?
Cy7 on bs-13297R-Cy7: excitation / emission 743nm/767nm.