ZO-1/TJP1 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1329r-apc-cy7-100uL |
| Product Name | ZO-1/TJP1 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Goat, GuineaPig |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZO-1 |
| Immunogen Range | 1551-1702/1733 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7082 |
| Swiss Prot | Q07157 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ZO-1; Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; TJP1; ZO1 |
| Background | The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZO-1/TJP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-1329R-APC-Cy7) validated for, and at what dilution?
bs-1329R-APC-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZO-1/TJP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-1329R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Goat, GuineaPig.
How should bs-1329R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZO-1/TJP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-1329R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human ZO-1 Immunogen range: 1551-1702/1733.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-1329R-APC-Cy7)?
APC-Cy7 on bs-1329R-APC-Cy7: excitation / emission 650nm/780nm.