GCAP1 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13306R-APC-Cy7-100uL |
| Product Name | GCAP1 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GCAP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2978 |
| Subcellular location | Cell membrane |
| Synonyms | COD3; GCAP 1; GCAP; Guanylate Cyclase Activating Protein Photoreceptor 1; Guanylate Cyclase Activating Protein Photoreceptor 1; Guanylate Cyclase Activator 1A; Guanylate Cyclase Activator 1A; Guanylin 1; Guanylin 1; guanylyl cyclase activating protein 1; Guanylyl cyclase-activating protein 1; GUC1A_HUMAN; GUCA; GUCA1; GUCA1A; GUCA1A. |
| Background | intracellular stimulation of guanylate cyclase (GC) by calcium, a key event in the recovery of the dark state of rod photoreceptors after exposure to light, is mediated by guanylate cyclase-activating protein (GCAP1). GCAPs are calcium-The binding proteins belonging to the calmodulin superfamily. GCAP1 is a calcium-binding protein that stimulates synthesis of c-GMP in photoreceptors. GCAP1 is present in rod and cone photoreceptor outer segments where phototransduction occurs. In contrast to other calcium-binding proteins from the calmodulin superfamily, the calcium-free form of GCAP1 stimulates the effector enzyme. By molecular cloning of human and mouse GCAP cDNA, the known mammalian GCAPs are found to be more than 90% similar, consisting of 201 to 205 amino acids, and containing three identically conserved calcium-binding sites. A related protein, GCAP2, is detectable only in the retina and results from a gene duplication event. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GCAP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-13306R-APC-Cy7) validated for, and at what dilution?
bs-13306R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GCAP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-13306R-APC-Cy7) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Horse.
How should bs-13306R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GCAP1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-13306R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human GCAP1
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-13306R-APC-Cy7)?
APC-Cy7 on bs-13306R-APC-Cy7: excitation / emission 650nm/780nm.