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GCHFR Polyclonal Antibody, HRP Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Pig
  • Chicken
Overview
Catalog # bs-13313R-HRP-100uL
Product Name GCHFR Polyclonal Antibody, HRP Conjugated
Applications
ELISA IHC-P IHC-F
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Pig, Chicken
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GCHFR
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2644
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms GCHFR; GFRP; GFRP_HUMAN; GTP cyclohydrolase 1 feedback regulatory protein; GTP cyclohydrolase I feedback regulator; GTP cyclohydrolase I feedback regulatory protein; HsT16933; MGC138467; MGC138469; p35.
Background GTP cyclohydrolase I feedback regulatory protein (GFRP) is encoded by the gene GCHFR. GFRP mediates feedback inhibition of GTP cyclohydrolase I activity by tetrahydrobiopterin. GFRP also acts as a mediator for the stimulatory effect of phenylalanine on enzyme activity. L-phenylalanine reverses this inhibition. The molecular weight of GFRP is approximately 9.5kDa although cross-linking experiments have shown that GFRP is usually found as a homodimer or as a pentamer. 15q15.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is GCHFR Polyclonal Antibody, HRP Conjugated (bs-13313R-HRP) validated for, and at what dilution?

bs-13313R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GCHFR Polyclonal Antibody, HRP Conjugated (bs-13313R-HRP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Chicken.

How should bs-13313R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GCHFR Polyclonal Antibody, HRP Conjugated (bs-13313R-HRP)?

KLH conjugated synthetic peptide derived from human GCHFR