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GCM2 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-13314R-HRP-100uL
Product Name GCM2 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Horse, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GCM2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9247
Swiss Prot O75603
Subcellular location Nucleus
Synonyms Chorion-specic transcription factor GCMb; GCM mot protein 2; GCMb; Glial cells missing homolog 2; glial cells missing homolog b; GCM2_HUMAN.
Background Glial cells missing homolog 2 (GCM2), also known as Chorion-specific transcription factor GCMb, is a 506 amino acid nuclear protein. GCM2 is a transcription factor that acts as an essential regulator of parathyroid development. GCM2 is also thought to mediate the effect of calcium on parathyroid hormone expression and secretion in parathyroid cells. GCM2 contains one N-terminal GCM domain, which has DNA binding activity. Mutations of the gene that encodes GCM2 are associated with hypoparathyroidism, an autosomal recessive condition characterized by hypocalcemia and hyperphosphatemia.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is GCM2 Polyclonal Antibody, HRP Conjugated (bs-13314R-HRP) validated for, and at what dilution?

bs-13314R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-13314R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GCM2 Polyclonal Antibody, HRP Conjugated (bs-13314R-HRP)?

KLH conjugated synthetic peptide derived from human GCM2