GCP6 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13321R-BF750-100uL |
| Product Name | GCP6 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GCP6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 85378 |
| Subcellular location | Cytoplasm |
| Synonyms | GCP 6; TUBGCP6; tubulin, gamma complex associated protein 6; GCP6_HUMAN. |
| Background | The g-Tubulin complex is composed of g Tubulin and the g-Tubulin complex-associated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. g-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the g-Tubulin complex. GCP6 (g-Tubulin complex component 6), also known as TUBGCP6, localizes to the centrosome and is a ubiquitously expressed 1,819 amino acid member of the g-Tubulin complex. Unlike GCP3 and GCP2, GCP6 is not well conserved among eukaryotes. Three isoforms of GCP6 exist due to alternative splicing events. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GCP6 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-13321R-BF750) validated for, and at what dilution?
bs-13321R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GCP6 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-13321R-BF750) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse.
How should bs-13321R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GCP6 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-13321R-BF750)?
KLH conjugated synthetic peptide derived from human GCP6
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-13321R-BF750)?
AbBy Fluor-750 on bs-13321R-BF750: excitation / emission 749nm/775nm.