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GCP6 Polyclonal Antibody, HRP Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-13321R-HRP
Product Name GCP6 Polyclonal Antibody, HRP Conjugated
Applications
ELISA IHC-P IHC-F
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Pig, Horse
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GCP6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 85378
Subcellular location Cytoplasm
Synonyms GCP 6; TUBGCP6; tubulin, gamma complex associated protein 6; GCP6_HUMAN.
Background The g-Tubulin complex is composed of g Tubulin and the g-Tubulin complex-associated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. g-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the g-Tubulin complex. GCP6 (g-Tubulin complex component 6), also known as TUBGCP6, localizes to the centrosome and is a ubiquitously expressed 1,819 amino acid member of the g-Tubulin complex. Unlike GCP3 and GCP2, GCP6 is not well conserved among eukaryotes. Three isoforms of GCP6 exist due to alternative splicing events.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is GCP6 Polyclonal Antibody, HRP Conjugated (bs-13321R-HRP) validated for, and at what dilution?

bs-13321R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GCP6 Polyclonal Antibody, HRP Conjugated (bs-13321R-HRP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse.

How should bs-13321R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GCP6 Polyclonal Antibody, HRP Conjugated (bs-13321R-HRP)?

KLH conjugated synthetic peptide derived from human GCP6