GDI1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13324R-Cy5 |
| Product Name | GDI1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Chimpanzee |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GDI1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2664 |
| Subcellular location | Cytoplasm |
| Synonyms | 1A; FLJ41411; GDI-1; gdi1; GDIA_HUMAN; GDIL; GDP dissociation inhibitor 1; Guanosine diphosphate dissociation inhibitor 1; Mental retardation X-linked 41; Mental retardation X-linked 48; MRX41; MRX48; Oligophrenin 2; Oligophrenin-2; OPHN2; Protein XAP 4; Protein XAP-4; Protein XAP4; Rab GDI alpha; Rab GDP dissociation inhibitor alpha; RABGD1A; RABGDIA; XAP 4. |
| Background | Rab proteins, a family of Ras-related small GTP-binding proteins, play a key role in regulating intracellular vesicle trafficking (1?). Rab GDP dissociation inhibitor (Rab GDI or GDI2) forms a soluble complex with Rab proteins and thereby prevents the exchange of GDP for GTP (1?). In mammals, there exist two major isoforms, Rab GDI alpha (also known as XAP-4) and Rab GDI beta (1,4). While the mammalian Rab GDI beta-genes are ubiquitously expressed, the Rab GDI ?genes are predominantly brain-specific (1). Since it is expressed predominantly in neural and sensory tissues, Rab GDI a may serve a specific function in neural signal transmission (5). The gene sequences for the Rab GDI proteins are extremely conserved in evolution, with substantial homology preserved across three eukaryotic kingdoms (5). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GDI1 Polyclonal Antibody, Cy5 Conjugated (bs-13324R-Cy5) validated for, and at what dilution?
bs-13324R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GDI1 Polyclonal Antibody, Cy5 Conjugated (bs-13324R-Cy5) react with?
Tested reactivity: Mouse, Chimpanzee. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Rabbit, Zebrafish.
How should bs-13324R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GDI1 Polyclonal Antibody, Cy5 Conjugated (bs-13324R-Cy5)?
KLH conjugated synthetic peptide derived from human GDI1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13324R-Cy5)?
Cy5 on bs-13324R-Cy5: excitation / emission 625,650nm/670nm.