GDPD1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13325R-Cy3 |
| Product Name | GDPD1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GDPD1/GDE4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 284161 |
| Subcellular location | Cytoplasm |
| Synonyms | 2610020H15Rik; GDE4; Glycerophosphodiester phosphodiesterase 4; glycerophosphodiester phosphodiesterase domain containing 1; RP23-352L3.1; GDPD1_HUMAN. |
| Background | GDE4 is a 314 amino acid cytoplasmic and multi-pass membrane protein that belongs to the glycerophosphoryl diester phosphodiesterase family. Expressed in small intestine, placenta, kidney, ovary, thymus, pancreas, spleen, liver and peripheral blood leukocytes, GDE4 contains one GDPD domain and exists as three alternatively spliced isoforms. GDE4 is encoded by a gene that maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GDPD1 Polyclonal Antibody, Cy3 Conjugated (bs-13325R-Cy3) validated for, and at what dilution?
bs-13325R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GDPD1 Polyclonal Antibody, Cy3 Conjugated (bs-13325R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Rabbit.
How should bs-13325R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GDPD1 Polyclonal Antibody, Cy3 Conjugated (bs-13325R-Cy3)?
KLH conjugated synthetic peptide derived from human GDPD1/GDE4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13325R-Cy3)?
Cy3 on bs-13325R-Cy3: excitation / emission 512,550nm/570,615nm.