Gemin 4 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13328R-FITC |
| Product Name | Gemin 4 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Gemin 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 50628 |
| Subcellular location | Cytoplasm |
| Synonyms | Gemin4; Gemin-4; Component of gems 4; Gem associated protein 4; HC56; HHRF 1; GEMI4_HUMAN. |
| Background | Gemin4 is a component of the SMN core complex which, while in the cytoplasm, plays an essential role in ribonucleoprotein (snRNP) assembly, including the biogenesis, delivery and recycling of snRNPs to the spliceosome. In the nucleus, where SMN is required for pre-mRNA splicing, Gemin4 concentrates next to coiled bodies in subnuclear structures called gems, that are highly enriched in splicosomal snRNPs, and in the nucleolus. Deletion or loss-of-function mutations in the SMN lead to the neurodegenerative disease spinal muscular atrophy (SMA). The human Gemin4 maps to chromosome 17p13. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Gemin 4 Polyclonal Antibody, FITC Conjugated (bs-13328R-FITC) validated for, and at what dilution?
bs-13328R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Gemin 4 Polyclonal Antibody, FITC Conjugated (bs-13328R-FITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Rabbit.
How should bs-13328R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Gemin 4 Polyclonal Antibody, FITC Conjugated (bs-13328R-FITC)?
KLH conjugated synthetic peptide derived from human Gemin 4
What are the excitation and emission wavelengths of the FITC conjugate (bs-13328R-FITC)?
FITC on bs-13328R-FITC: excitation / emission 494nm/518nm.