Gemin 7 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13330R-Cy3-100uL |
| Product Name | Gemin 7 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Gemin 7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79760 |
| Subcellular location | Nucleus |
| Synonyms | SIP3; Gemin7; Gemin-7; GEMI7_HUMAN. |
| Background | Gemin7 is a 131 amino acid protein encoded by the mouse gene Gemin7. Gemin7, along with Gemin6, is a significant component of the the large multiprotein human SMN complex. The SMN complex functions as an assembly machine for small nuclear ribonucleoproteins (snRNPs)-the major components of the spliceosome. The survival of motor neurons (SMN) protein, a product of the disease gene of the common neurodegenerative disease, spinal muscular atrophy, is also part of the SMN complex. Although Gemin6 and Gemin7 have no significant sequence similarity with Sm proteins, both adopt canonical Sm folds. Moreover, Gemin6 and Gemin7 exist as a heterodimer, and interact with each other via an interface similar to that which mediates interactions among the Sm proteins. The Gemin6/Gemin7 complex binds to Sm proteins and might help organize Sm proteins for formation of Sm rings on snRNA targets. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Gemin 7 Polyclonal Antibody, Cy3 Conjugated (bs-13330R-Cy3) validated for, and at what dilution?
bs-13330R-Cy3 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Gemin 7 Polyclonal Antibody, Cy3 Conjugated (bs-13330R-Cy3) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Horse, Chicken.
How should bs-13330R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Gemin 7 Polyclonal Antibody, Cy3 Conjugated (bs-13330R-Cy3)?
KLH conjugated synthetic peptide derived from human Gemin 7
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13330R-Cy3)?
Cy3 on bs-13330R-Cy3: excitation / emission 512,550nm/570,615nm.