Gemin 6 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13333R-BF680-100uL |
| Product Name | Gemin 6 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Gemin 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79833 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | FLJ23459; Gemin6; Gemin-6; Gem nuclear organelle associated protein 6; Gem-associated protein 6; GEMI6; GEMI6_HUMAN; Gemin-6; GEMIN6; SIP2. |
| Background | Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord. SMA is caused by deletion or loss-of-function mutations in the SMN (Survival of Motor Neuron) gene. Gemin6, the protein product of human chromosome 2p22.2, associates directly with SMN and is a part of the SMN complex containing Gemin2, Gemin3, Gemin4 and Gemin5 as well as several spliceosomal snRNP proteins. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus. The SMN complex is found in both the cytoplasm and the nucleus. The nuclear form is concentrated in subnuclear bodies called gems (Gemini of the coiled bodies). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Gemin 6 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13333R-BF680) validated for, and at what dilution?
bs-13333R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Gemin 6 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13333R-BF680) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Horse, Rabbit.
How should bs-13333R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Gemin 6 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13333R-BF680)?
KLH conjugated synthetic peptide derived from human Gemin 6
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-13333R-BF680)?
AbBy Fluor-680 on bs-13333R-BF680: excitation / emission 679nm/702nm.