TFAR19/PDCD5 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1333R-Cy3-100uL |
| Product Name | TFAR19/PDCD5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TFAR19 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9141 |
| Subcellular location | Cytoplasm, Nucleus, Secreted |
| Synonyms | TF-1 cell apoptsis-related gene 19; Programmed cell death protein 5; TF-1 cell apoptosis-related protein 19; Protein TFAR19; PDCD5. |
| Background | This gene encodes a protein expressed in tumor cells during apoptosis independent of the apoptosis-inducing stimuli. Prior to apoptosis induction, this gene product is distributed in both the nucleus and cytoplasm. Once apoptosis is induced, the level of this protein increases and by relocation from the cytoplasm, it accumulates in the nucleus. Although its exact function is not defined, this protein is thought to play an early and universal role in apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TFAR19/PDCD5 Polyclonal Antibody, Cy3 Conjugated (bs-1333R-Cy3) validated for, and at what dilution?
bs-1333R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TFAR19/PDCD5 Polyclonal Antibody, Cy3 Conjugated (bs-1333R-Cy3) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Chicken.
How should bs-1333R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TFAR19/PDCD5 Polyclonal Antibody, Cy3 Conjugated (bs-1333R-Cy3)?
KLH conjugated synthetic peptide derived from human TFAR19
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1333R-Cy3)?
Cy3 on bs-1333R-Cy3: excitation / emission 512,550nm/570,615nm.