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MTERF1 Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-13340R-APC-Cy7-100uL
Product Name MTERF1 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MTERF1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7978
Subcellular location Cytoplasm
Synonyms mitochondrial; Mitochondrial transcription termination factor 1; MTERF1; mTERF; MTERF_HUMAN; Transcription termination factor.
Background Members of the mTERF family, including MTERF, MTERFD1, MTERFD2 and MTERFD3, are mitochondrial proteins that are believed to be transcription termination factors. MTERF (mitochondrial transcription termination factor 1) is composed of 399 amino acids and contains three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It has been suggested that only the phosphorylated form of MTERF has transcription termination activity. MTERFD1 is also thought to act as a mitochondrial transcription regulator and is expressed as two isoforms produced by alternative splicing. MTERFD3 is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription. MTERFD3 is expressed in heart, skeletal muscle, pancreas and liver.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MTERF1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-13340R-APC-Cy7) validated for, and at what dilution?

bs-13340R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-13340R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MTERF1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-13340R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human MTERF1

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-13340R-APC-Cy7)?

APC-Cy7 on bs-13340R-APC-Cy7: excitation / emission 650nm/780nm.

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