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GGCT Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Pig
Overview
Catalog # bs-13346R-Cy5
Product Name GGCT Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Pig
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GGCT
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms C7orf24; CRF21; Cytochrome c-releasing factor 21; Gamma-glutamylcyclotransferase; Ggct; GGCT_HUMAN.
Background Catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides and may play a significant role in glutathione homeostasis. Induces release of cytochrome c from mitochondria with resultant induction of apoptosis.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GGCT Polyclonal Antibody, Cy5 Conjugated (bs-13346R-Cy5) validated for, and at what dilution?

bs-13346R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-13346R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GGCT Polyclonal Antibody, Cy5 Conjugated (bs-13346R-Cy5)?

KLH conjugated synthetic peptide derived from human GGCT

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13346R-Cy5)?

Cy5 on bs-13346R-Cy5: excitation / emission 625,650nm/670nm.