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Giantin Polyclonal Antibody

Hela cells probed with Giantin\/GM130 Polyclonal Antibody, Unconjugated (bs-13356R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Hela cells probed with Giantin\/GM130 Polyclonal Antibody, Unconjugated (bs-13356R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Giantin\/GOLGB1 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Giantin\/GOLGB1 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human cervix cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with\\tGiantin\/GOLGB1 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human cervix cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with\\tGiantin\/GOLGB1 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Giantin Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Giantin Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Lovo cells probed with Giantin\/GOLGB1 Polyclonal Antibody, ALEXA FLUOR\u00ae 488 Conjugated (bs-13356R-A488) at 1:20 for 30 minutes compared to control cells (blue) and isotype control (orange).
Lovo cells probed with Giantin\/GOLGB1 Polyclonal Antibody, ALEXA FLUOR\u00ae 488 Conjugated (bs-13356R-A488) at 1:20 for 30 minutes compared to control cells (blue) and isotype control (orange).
Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Giantin\/GOLGB1 Polyclonal Antibody, ALEXA FLUOR\u00ae 488 Conjugated (bs-13356R-AF488)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Giantin\/GOLGB1 Polyclonal Antibody, ALEXA FLUOR\u00ae 488 Conjugated (bs-13356R-AF488)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Horse
  • Rabbit
  • Monkey
  • Hamster
Overview
Catalog # bs-13356R-100uL
Product Name Giantin Polyclonal Antibody
Applications
WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Horse, Rabbit, Monkey, Hamster
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Giantin/GOLGB1
Immunogen Range 751-850/3259
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2804
Swiss Prot Q14789
Subcellular location Cytoplasm, Cell membrane
Synonyms GCP; GCP372; GOLIM1; Golgin subfamily B member 1; 372 kDa Golgi complex-associated protein; Giantin; Macrogolgin; GOLGB1
Background May participate in forming intercisternal cross-bridges of the Golgi complex.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Giantin Polyclonal Antibody (bs-13356R) validated for, and at what dilution?

bs-13356R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Giantin Polyclonal Antibody (bs-13356R) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Horse, Rabbit, Monkey, Hamster.

How should bs-13356R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Giantin Polyclonal Antibody (bs-13356R)?

KLH conjugated synthetic peptide derived from human Giantin/GOLGB1 Immunogen range: 751-850/3259.

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