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GLS2 Polyclonal Antibody, FITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-13376R-FITC
Product Name GLS2 Polyclonal Antibody, FITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GLS2/LGA
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 27165
Swiss Prot Q9UI32
Subcellular location Cytoplasm
Synonyms GA; GLS; LGA; hLGA; Glutaminase liver isoform, mitochondrial; L-glutaminase; L-glutamine amidohydrolase; GLS2
Background Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GLS2 Polyclonal Antibody, FITC Conjugated (bs-13376R-FITC) validated for, and at what dilution?

bs-13376R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GLS2 Polyclonal Antibody, FITC Conjugated (bs-13376R-FITC) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-13376R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GLS2 Polyclonal Antibody, FITC Conjugated (bs-13376R-FITC)?

KLH conjugated synthetic peptide derived from human GLS2/LGA

What are the excitation and emission wavelengths of the FITC conjugate (bs-13376R-FITC)?

FITC on bs-13376R-FITC: excitation / emission 494nm/518nm.