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GLT25D2 Polyclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-13379R-BF594-100uL
Product Name GLT25D2 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GLT25D2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23127
Subcellular location Cytoplasm
Synonyms C1orf17; Glt25d2; Glycosyltransferase 25 family member 2; GT252_HUMAN; Hydroxylysine galactosyltransferase 2; Procollagen galactosyltransferase 2; RP11-498P10.2.
Background GLT25D2 is a 626 amino acid protein that localizes to endoplasmic reticulum lumen and belongs to the glycosyltransferase 25 family. Possessing beta-galactosyltransferase activity, GLT25D2 transports beta-galactose to hydroxylysine residues on collagen but lacks glucosyltransferase activity. GLT25D2 is expressed in skeletal muscle and brain, and is encoded by a gene located on human chromosome 1q25.3, a region associated with the hereditary prostate cancer (HPC1) locus. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson?s disease, Gaucher disease, schizophrenia and Usher syndrome.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GLT25D2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13379R-BF594) validated for, and at what dilution?

bs-13379R-BF594 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GLT25D2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13379R-BF594) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig, Horse, Chicken.

How should bs-13379R-BF594 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GLT25D2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13379R-BF594)?

KLH conjugated synthetic peptide derived from human GLT25D2

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-13379R-BF594)?

AbBy Fluor-594 on bs-13379R-BF594: excitation / emission 590nm/617nm.

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