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GLT25D2 Polyclonal Antibody, Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-13379R-Cy7
Product Name GLT25D2 Polyclonal Antibody, Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GLT25D2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23127
Subcellular location Cytoplasm
Synonyms C1orf17; Glt25d2; Glycosyltransferase 25 family member 2; GT252_HUMAN; Hydroxylysine galactosyltransferase 2; Procollagen galactosyltransferase 2; RP11-498P10.2.
Background GLT25D2 is a 626 amino acid protein that localizes to endoplasmic reticulum lumen and belongs to the glycosyltransferase 25 family. Possessing beta-galactosyltransferase activity, GLT25D2 transports beta-galactose to hydroxylysine residues on collagen but lacks glucosyltransferase activity. GLT25D2 is expressed in skeletal muscle and brain, and is encoded by a gene located on human chromosome 1q25.3, a region associated with the hereditary prostate cancer (HPC1) locus. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes, comprises nearly 8% of the human genome and houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson?s disease, Gaucher disease, schizophrenia and Usher syndrome.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GLT25D2 Polyclonal Antibody, Cy7 Conjugated (bs-13379R-Cy7) validated for, and at what dilution?

bs-13379R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GLT25D2 Polyclonal Antibody, Cy7 Conjugated (bs-13379R-Cy7) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig, Horse, Chicken.

How should bs-13379R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GLT25D2 Polyclonal Antibody, Cy7 Conjugated (bs-13379R-Cy7)?

KLH conjugated synthetic peptide derived from human GLT25D2

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13379R-Cy7)?

Cy7 on bs-13379R-Cy7: excitation / emission 743nm/767nm.