Phospho-Glucocorticoid Receptor (Ser226) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13384R-Biotin-100uL |
| Product Name | Phospho-Glucocorticoid Receptor (Ser226) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Glucocorticoid Receptor around the phosphorylation site of Ser226 |
| Modification Site | Ser226 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 2908 |
| Swiss Prot | P04150 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Glucocorticoid Receptor phospho S226; p-Glucocorticoid Receptor phospho S226; Glucocorticoid Receptor; GCCR; GCR; GR; Nuclear receptor subfamily 3 group C member 1; Glucocorticoid receptor lymphocyte; GRL; Grl1; Nr3c1; NR3C1. |
| Background | Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Phospho-Glucocorticoid Receptor (Ser226) Polyclonal Antibody, Biotin Conjugated (bs-13384R-Biotin) validated for, and at what dilution?
bs-13384R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Glucocorticoid Receptor (Ser226) Polyclonal Antibody, Biotin Conjugated (bs-13384R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-13384R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Glucocorticoid Receptor (Ser226) Polyclonal Antibody, Biotin Conjugated (bs-13384R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human Glucocorticoid Receptor around the phosphorylation site of Ser226