Glucose 6 phosphatase 2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13386R-Cy5-100uL |
| Product Name | Glucose 6 phosphatase 2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Glucose 6 phosphatase 2/IGRP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57818 |
| Subcellular location | Cell membrane |
| Synonyms | G 6 Pase 2; G 6 Pase2; G6Pase 2; G6Pase2; G6PC 2; G6pc rs; G6pcrs; Glucose 6 phosphatase 2; Glucose 6 phosphatase, catalytic, 2; Glucose 6 phosphatase, catalytic, related; Glucose 6 phosphate catalytic 2; Islet specic G6CP related protein; Islet specic glucose 6 phosphatase; Islet specic glucose 6 phosphatase catalytic subunit related protein; G6PC2_HUMAN. |
| Background | Glucose-6-phosphatase (G6Pase), is a multicomponent enzyme system that hydrolyzes glucose-6-phosphate in the final step of gluconeogenesis and gluconeolysis. G6Pase localizes to the endoplasmic reticulum, and while liver, kidney, and intestine are the only tissues that express the first identified isoform, G6Pase-alpha, a second form, designated G6Pase-, contributes to blood glucose homeostasis in a wider range of tissues. Islet-specific G-6-Pase catalytic subunit-related protein (IGRP), a homolog of the catalytic subunit of G6Pase, may play a role in the regulation of islet metabolism and in insulin secretion induced by metabolites. The exact catalytic acivity of IGRP is not defined. Identification of inhibitors of IGRP have potential therapeutic benefits for treatment of type 2 diabetes resulting from insulin secretion defects. Structurally, IGRP has been shown to be a glycoprotein held in the endoplasmic reticulum by nine transmembrane domains, which are then degraded in cells through the proteasome pathway generating MHC class I presented peptides. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Glucose 6 phosphatase 2 Polyclonal Antibody, Cy5 Conjugated (bs-13386R-Cy5) validated for, and at what dilution?
bs-13386R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Glucose 6 phosphatase 2 Polyclonal Antibody, Cy5 Conjugated (bs-13386R-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Horse, Rabbit.
How should bs-13386R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Glucose 6 phosphatase 2 Polyclonal Antibody, Cy5 Conjugated (bs-13386R-Cy5)?
KLH conjugated synthetic peptide derived from human Glucose 6 phosphatase 2/IGRP
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13386R-Cy5)?
Cy5 on bs-13386R-Cy5: excitation / emission 625,650nm/670nm.