GLUD2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13390R-BF350-100uL |
| Product Name | GLUD2 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GLUD2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2747 |
| Subcellular location | Cytoplasm |
| Synonyms | GDH 2; GDH; GDH2; GLUDP1; Glutamate dehydrogenase 2; Glutamate dehydrogenase 2 mitochondrial; Glutamate dehydrogenase pseudogene 1. |
| Background | GLUD2 is both mitochondrial matrix enzymes belonging to the Glu/Leu/Phe/Val dehydrogenases family. Exisiting as homohexamers, GLUD1 catalyzes the oxidative deamination of glutamate to ?ketoglutarate and ammonia while GLUD2 is involved in the recycling of glutamate during neurotransmission. GLUD1 is critical for regulating amino acid induced insulin secretion and is allosterically activated by ADP and inhibited by GTP and ATP. Mutations in the gene encoding GLUD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLUD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. GLUD2 is expressed in testis and retina, with lower levels found in brain. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GLUD2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-13390R-BF350) validated for, and at what dilution?
bs-13390R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13390R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GLUD2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-13390R-BF350)?
KLH conjugated synthetic peptide derived from human GLUD2
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-13390R-BF350)?
AbBy Fluor-350 on bs-13390R-BF350: excitation / emission 346nm/442nm.