NRF1 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1342R-FITC |
| Product Name | NRF1 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NRF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4899 |
| Swiss Prot | Q16656 |
| Subcellular location | Nucleus |
| Synonyms | ALPHA-PAL; Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein; NRF1 |
| Background | Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NRF1 Polyclonal Antibody, FITC Conjugated (bs-1342R-FITC) validated for, and at what dilution?
bs-1342R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NRF1 Polyclonal Antibody, FITC Conjugated (bs-1342R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1342R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NRF1 Polyclonal Antibody, FITC Conjugated (bs-1342R-FITC)?
KLH conjugated synthetic peptide derived from human NRF1
What are the excitation and emission wavelengths of the FITC conjugate (bs-1342R-FITC)?
FITC on bs-1342R-FITC: excitation / emission 494nm/518nm.