Ezrin/Radixin Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1343R-FITC |
| Product Name | Ezrin/Radixin Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Ezrin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7430 |
| Swiss Prot | P15311 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CVL; CVIL; VIL2; HEL-S-15; Ezrin; Cytovillin; Villin-2; p81; EZR |
| Background | Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Ezrin/Radixin Polyclonal Antibody, FITC Conjugated (bs-1343R-FITC) validated for, and at what dilution?
bs-1343R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Ezrin/Radixin Polyclonal Antibody, FITC Conjugated (bs-1343R-FITC) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Rabbit, GuineaPig.
How should bs-1343R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Ezrin/Radixin Polyclonal Antibody, FITC Conjugated (bs-1343R-FITC)?
KLH conjugated synthetic peptide derived from human Ezrin
What are the excitation and emission wavelengths of the FITC conjugate (bs-1343R-FITC)?
FITC on bs-1343R-FITC: excitation / emission 494nm/518nm.