GM2A Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13452R-Cy3 |
| Product Name | GM2A Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GM2A/SAP3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2760 |
| Swiss Prot | P17900 |
| Subcellular location | Cytoplasm |
| Synonyms | SAP-3; GM2-AP; Ganglioside GM2 activator; Cerebroside sulfate activator protein; Sphingolipid activator protein 3; GM2A |
| Background | The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity (By similarity). Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GM2A Polyclonal Antibody, Cy3 Conjugated (bs-13452R-Cy3) validated for, and at what dilution?
bs-13452R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GM2A Polyclonal Antibody, Cy3 Conjugated (bs-13452R-Cy3) react with?
Tested reactivity: Human, Rat.
How should bs-13452R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GM2A Polyclonal Antibody, Cy3 Conjugated (bs-13452R-Cy3)?
KLH conjugated synthetic peptide derived from human GM2A/SAP3
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13452R-Cy3)?
Cy3 on bs-13452R-Cy3: excitation / emission 512,550nm/570,615nm.