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GMPPA Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-13460R-Cy7
Product Name GMPPA Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GMPPA
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms GDP mannose pyrophosphorylase A; GTP mannose 1 phosphate guanylyltransferase alpha; Mannose 1 phosphate guanylyltransferase GDP;GMPPA_HUMAN.
Background GMPPA is a GDP-mannose pyrophosphorylase which catalyzes the conversion of mannose-1-phosphate and GTP to GDP-mannose, in the production of N-linked oligosaccharides.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GMPPA Polyclonal Antibody, Cy7 Conjugated (bs-13460R-Cy7) validated for, and at what dilution?

bs-13460R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-13460R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GMPPA Polyclonal Antibody, Cy7 Conjugated (bs-13460R-Cy7)?

KLH conjugated synthetic peptide derived from human GMPPA

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13460R-Cy7)?

Cy7 on bs-13460R-Cy7: excitation / emission 743nm/767nm.