GNPDA2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13474R-Cy5 |
| Product Name | GNPDA2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GNPDA2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 132789 |
| Subcellular location | Cytoplasm |
| Synonyms | GlcN6P deaminase 2; Glucosamine 6 phosphate deaminase 2; Glucosamine 6 phosphate isomerase 2; Glucosamine 6 phosphate isomerase SB52; Glucosamine-6-phosphate deaminase 2; Glucosamine-6-phosphate isomerase 2; Glucosamine-6-phosphate isomerase SB52; GNP2; GNPDA 2; Gnpda2; GNPI2_HUMAN; SB52. |
| Background | During fertilization in mammals, the sperm activates the egg by causing an increase in the level of free cytoplasmic calcium concentration. This increased calcium concentration induces a characteristic series of oscillations that trigger egg activation and early embryo development. A hamster protein named oscillin is thought to be involved in this pathway. The enzyme glucosamine-6-phosphate isomerase (GNPI) or deaminase (GNPDA1) and the related protein GNPDA2 are the human homologs of hamster oscillin. GNPDA1 and GNPDA2 catalyze the conversion of GNP to fructose-6-phosphate and ammonia. Both proteins exist as homohexamers and are ubiquitously expressed with highest expression in testis, ovary and heart. Three isoforms of GNPDA2 are expressed due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GNPDA2 Polyclonal Antibody, Cy5 Conjugated (bs-13474R-Cy5) validated for, and at what dilution?
bs-13474R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GNPDA2 Polyclonal Antibody, Cy5 Conjugated (bs-13474R-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse.
How should bs-13474R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GNPDA2 Polyclonal Antibody, Cy5 Conjugated (bs-13474R-Cy5)?
KLH conjugated synthetic peptide derived from human GNPDA2
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13474R-Cy5)?
Cy5 on bs-13474R-Cy5: excitation / emission 625,650nm/670nm.