phospho-Rb (Ser780) Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1347R-BF350-100uL |
| Product Name | phospho-Rb (Ser780) Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Rb around the phosphorylation site of (Ser780) |
| Modification Site | Ser780 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5925 |
| Subcellular location | Nucleus |
| Synonyms | RbSer780; OSRC; P105 RB; P105RB; PP105; PP110; pRb; RB 1; RB1; RB1 protein; Retinoblastoma 1 including osteosarcoma; Retinoblastoma 1; Retinoblastoma associated protein; Including osteosarcoma; Osteosarcoma; p105-Rb; Rb; RB_HUMAN; Retinoblastoma suspectibility protein; Retinoblastoma-associated protein; Retinoblastoma related osteosarcoma; Retinoblastoma susceptibility gene. |
| Background | Nuclear Marker.The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Rb (Ser780) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1347R-BF350) validated for, and at what dilution?
bs-1347R-BF350 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Rb (Ser780) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1347R-BF350) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Chicken.
How should bs-1347R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Rb (Ser780) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1347R-BF350)?
KLH conjugated synthetic phosphopeptide derived from human Rb around the phosphorylation site of (Ser780)
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-1347R-BF350)?
AbBy Fluor-350 on bs-1347R-BF350: excitation / emission 346nm/442nm.