GOLGA7 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13484R-Cy7 |
| Product Name | GOLGA7 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Sheep |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GOLGA7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51125 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | GCP 16; GCP16; GOLGA 7; GOLGA3AP1; GOLGA7A; Golgi autoantigen golgin subfamily a 7; Golgi complex associated protein of 16kDa; Golgin A7; Golgin subfamily A member 7; HSPC041; MGC21096; MGC4876; GOGA7_HUMAN. |
| Background | GOLGA7 is a multi-pass membrane protein belonging to the Erf4 family of proteins. It is the functional ortholog of the yeast Erf4 protein. Localizing to the Golgi apparatus, GOLGA7 is a widely expressed protein but its expression is absent from colon and thymus tissues. GOLGA7 is palmitoylated on two cysteine residues, and this palmitoylation is required for its interaction with golgin 160 and its Golgi-localization. GOLGA7 also forms a complex with ZDHHC9 and, together, these proteins function as a Ras palmitoyltransferase (Ras PAT) which is required for palmitoylation of H-Ras and N-Ras proteins. The palmitoylation of Ras proteins is essential for the trafficking of Ras proteins from the Golgi to the plasma membrane, thus implicating GOLGA7 in protein transport from the Golgi to the cell surface. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GOLGA7 Polyclonal Antibody, Cy7 Conjugated (bs-13484R-Cy7) validated for, and at what dilution?
bs-13484R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GOLGA7 Polyclonal Antibody, Cy7 Conjugated (bs-13484R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Sheep.
How should bs-13484R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GOLGA7 Polyclonal Antibody, Cy7 Conjugated (bs-13484R-Cy7)?
KLH conjugated synthetic peptide derived from human GOLGA7
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13484R-Cy7)?
Cy7 on bs-13484R-Cy7: excitation / emission 743nm/767nm.