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Golgin subfamily A member 4 Polyclonal Antibody, FITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-13487R-FITC
Product Name Golgin subfamily A member 4 Polyclonal Antibody, FITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Cow, Pig, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Golgin subfamily A member 4/Golgin 245
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2803
Subcellular location Cytoplasm, Cell membrane
Synonyms 256 kDa golgin; 72.1 protein; GCP2; GOGA4_HUMAN; GOLG; GOLGA4; golgi autoantigen, golgin subfamily a, 4; Golgin subfamily A member 4; golgin-240; Golgin-245; MU-RMS-40.18; OTTHUMP00000161363; OTTHUMP00000208897; OTTHUMP00000209805; p230; Protein 72.1; Trans-Golgi p230.
Background Golgin 245 is also known as p230, GCP2, GOLG or golgi autoantigen and is a 2,230 amino acid protein that is expressed as three isoforms. Golgin 245 is localized to the cytoplasm in cells and is a member of the golgin family, all of which are peripheral membrane proteins associated with the Golgi complex. Golgin 245 has a carboxyl-terminal GRIP domain, which attaches to the trans-Golgi network (TGN) and TGN-derived vesicles. It is thought that the interaction that takes place between golgin 245 and MACF1, which cross-links microtubules to the Actin cytoskeleton, allows proteins to be transported from the TGN to the cell periphery. against golgin 245 are associated with two chronic disorders known as Sjren's syndrome and Hepatitis B.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Golgin subfamily A member 4 Polyclonal Antibody, FITC Conjugated (bs-13487R-FITC) validated for, and at what dilution?

bs-13487R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Golgin subfamily A member 4 Polyclonal Antibody, FITC Conjugated (bs-13487R-FITC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Pig, Horse, Rabbit.

How should bs-13487R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Golgin subfamily A member 4 Polyclonal Antibody, FITC Conjugated (bs-13487R-FITC)?

KLH conjugated synthetic peptide derived from human Golgin subfamily A member 4/Golgin 245

What are the excitation and emission wavelengths of the FITC conjugate (bs-13487R-FITC)?

FITC on bs-13487R-FITC: excitation / emission 494nm/518nm.