GORASP2 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13492R-APC-Cy7-100uL |
| Product Name | GORASP2 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig, Horse, Rabbit, Zebrafish, Xenopuslaevis |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Golgi phosphoprotein 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26003 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Golgi phosphoprotein 6; Golgi reassembly stacking protein 2; Golgi reassembly stacking protein 2, 55kDa; Golgi reassembly stacking protein of 55 kDa; Golgi reassembly-stacking protein 2; Golgi reassembly-stacking protein of 55 kDa; GOLPH2; GOLPH6; GORASP2; GORS2_HUMAN; GRASP55; GRS2; p59; RP23-428O15.1. |
| Background | The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, the golgins and other peripheral membrane components such as GRASP55 and GRASP65 (1). GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery. GRASP55 is highly homologous to GRASP65 and contains two PDZ domains. GRASP55 is myristoylated and palmitoylated. Unlike GRASP65, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi. Both GRASP55 and GRASP65 function in the stacking of Golgi Cisternae (2,3). The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure (4). ERK2 directly phosphorylates GRASP55, which is phosphorylated in mitotic cells, suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis (5). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GORASP2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-13492R-APC-Cy7) validated for, and at what dilution?
bs-13492R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GORASP2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-13492R-APC-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Horse, Rabbit, Zebrafish, Xenopuslaevis.
How should bs-13492R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GORASP2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-13492R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human Golgi phosphoprotein 6
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-13492R-APC-Cy7)?
APC-Cy7 on bs-13492R-APC-Cy7: excitation / emission 650nm/780nm.