GPBP1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13503R-Cy5 |
| Product Name | GPBP1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GPBP1/Vasculin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 65056 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DKFZp761C169; GC rich promoter binding protein 1; GC-rich promoter-binding protein 1; GPBP; Gpbp1; GPBP1_HUMAN; MGC126339; SSH6; Vascular wall linked; Vascular wall-linked protein; Vasculin. |
| Background | Vascular wall-linked protein, or Vasculin, is primarily expressed in the arterial wall and in plasma. It is also differentially expressed in human atherogenesis. Alternative splicing of exon 3 of the Vasculin gene produces three variants. Vasculin binds to and activates the minimal self-sufficient promoter element (MSPE) of the mouse Ada gene promoter and binds to and partially suppresses the GC-rich promoter of the nonhomologous human TOP2A gene promoter. It acts as a nuclear factor that can form complexes with TATA-binding proteins, transcription factors TFIIB and TFIIF, RNA polymerase II and p300. The regulated expression of Vasculin in plaques suggests that it may be involved in atherogenesis, and its presence in plasma may implicate Vasculin as a marker for atherosclerosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GPBP1 Polyclonal Antibody, Cy5 Conjugated (bs-13503R-Cy5) validated for, and at what dilution?
bs-13503R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPBP1 Polyclonal Antibody, Cy5 Conjugated (bs-13503R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-13503R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPBP1 Polyclonal Antibody, Cy5 Conjugated (bs-13503R-Cy5)?
KLH conjugated synthetic peptide derived from human GPBP1/Vasculin
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13503R-Cy5)?
Cy5 on bs-13503R-Cy5: excitation / emission 625,650nm/670nm.