DAP5 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1350R-Cy5.5-100uL |
| Product Name | DAP5 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DAP-5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1982 |
| Subcellular location | Cytoplasm |
| Synonyms | Death Associated Protein-5; Death associated protein 5; Eukaryotic translation initiation factor 4 gamma 2; Nat1; Novel APOBEC-1 target 1; Translation repressor NAT1. |
| Background | Death Associated Protein 5 (DAP5) is a 97 kDa protein with high amino acid sequence homology to Eukaryotic Translation Initiation Factor 4G (eIF4G). Compared with eIF4G, DAP5 lacks the N-terminal region necessary for cap-dependent translation and has a unique C-terminal part functioning as a regulator for interferon-gamma induced cell death. During apoptosis, DAP5 is cleaved at Asp790. The C-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DAP5 Polyclonal Antibody, Cy5.5 Conjugated (BS-1350R-CY5-5) validated for, and at what dilution?
BS-1350R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DAP5 Polyclonal Antibody, Cy5.5 Conjugated (BS-1350R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should BS-1350R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DAP5 Polyclonal Antibody, Cy5.5 Conjugated (BS-1350R-CY5-5)?
KLH conjugated synthetic peptide derived from human DAP-5
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-1350R-CY5-5)?
Cy5.5 on BS-1350R-CY5-5: excitation / emission 675nm/694nm.