GPR101 Polyclonal Antibody, Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13511R-Cy7 |
| Product Name | GPR101 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human G protein coupled receptor 101 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83550 |
| Subcellular location | Cell membrane |
| Synonyms | G protein-coupled receptor 101; G protein coupled receptor 101; GP101_HUMAN; GPCR6; GPR101; Probable G-protein coupled receptor 101; GPCR GPR101. |
| Background | G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR101 (G protein-coupled receptor 101), also known as GPCR6, is a 508 amino acid multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. GPR101 functions as an orphan receptor that is thought to play a role in signaling events throughout the cell. Expressed predominantly in brain, GPR101 participates in a wide range of activities in the CNS via modulation of cAMP levels. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GPR101 Polyclonal Antibody, Cy7 Conjugated (bs-13511R-Cy7) validated for, and at what dilution?
bs-13511R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13511R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPR101 Polyclonal Antibody, Cy7 Conjugated (bs-13511R-Cy7)?
KLH conjugated synthetic peptide derived from human G protein coupled receptor 101
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13511R-Cy7)?
Cy7 on bs-13511R-Cy7: excitation / emission 743nm/767nm.