GPR124 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13515R-Cy3-100uL |
| Product Name | GPR124 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human G protein coupled receptor 124 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 25960 |
| Swiss Prot | Q96PE1 |
| Subcellular location | Cell membrane |
| Synonyms | DKFZp434C211; DKFZp434J0911; FLJ14390; G protein coupled receptor 124; G-protein coupled receptor 124; GP124_HUMAN; GPR 124; GPR124; KIAA1531; TEM 5; TEM5; Tumor endothelial marker 5; Tumor endothelial marker 5 precursor; GPCR GPR124; Adhesion G protein-coupled receptor A2; ADGRA2. |
| Background | Tumor endothelial markers (TEMs) are abundantly expressed in the blood vessels of human solid tumors during angiogenesis and neoangiogensis. These include TEM1 (endosialin), TEM5 (G-protein coupled receptor 124) and TEM7 (plexin domain containing 1). TEMs are associated with the cell surface membrane at low levels in normal human and mouse tissues. TEM5 is a seven-pass transmembrane receptor, whereas TEM1, TEM7 and TEM8 span the membrane once. TEM5 expression is elevated during tumor angiogenesis and neoangiogenesis. TEM7 is highly expressed in tumor endothelium and neurons. Therefore, TEM5 and TEM7 may be suitable targets for the development of antiangiogenic therapies. |
| Application Dilution | |
| WB | IFIHC-P1:50-200 |
| IF | IHC-F1:50-200 |
Frequently asked questions
Which applications is GPR124 Polyclonal Antibody, Cy3 Conjugated (bs-13515R-Cy3) validated for, and at what dilution?
bs-13515R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPR124 Polyclonal Antibody, Cy3 Conjugated (bs-13515R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse.
How should bs-13515R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPR124 Polyclonal Antibody, Cy3 Conjugated (bs-13515R-Cy3)?
KLH conjugated synthetic peptide derived from human G protein coupled receptor 124
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13515R-Cy3)?
Cy3 on bs-13515R-Cy3: excitation / emission 512,550nm/570,615nm.