GPR124 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13515R-100uL |
| Product Name | GPR124 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human G protein coupled receptor 124 |
| Immunogen Range | 351-450/1338 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 25960 |
| Swiss Prot | Q96PE1 |
| Subcellular location | Cell membrane |
| Synonyms | DKFZp434C211; DKFZp434J0911; FLJ14390; G protein coupled receptor 124; G-protein coupled receptor 124; GP124_HUMAN; GPR 124; GPR124; KIAA1531; TEM 5; TEM5; Tumor endothelial marker 5; Tumor endothelial marker 5 precursor; GPCR GPR124; Adhesion G protein-coupled receptor A2; ADGRA2. |
| Background | Tumor endothelial markers (TEMs) are abundantly expressed in the blood vessels of human solid tumors during angiogenesis and neoangiogensis. These include TEM1 (endosialin), TEM5 (G-protein coupled receptor 124) and TEM7 (plexin domain containing 1). TEMs are associated with the cell surface membrane at low levels in normal human and mouse tissues. TEM5 is a seven-pass transmembrane receptor, whereas TEM1, TEM7 and TEM8 span the membrane once. TEM5 expression is elevated during tumor angiogenesis and neoangiogenesis. TEM7 is highly expressed in tumor endothelium and neurons. Therefore, TEM5 and TEM7 may be suitable targets for the development of antiangiogenic therapies. |
| Application Dilution | |
| WB | ELISA1:500-1000 |
| ELISA | IHC-P1:200-400 |
| IHC-P | IHC-F1:100-500 |
| IHC-F | IFIHC-P1:50-200 |
| IF | IHC-F1:50-200 |
| ICC/IF | IFICC1:50-200 |
Frequently asked questions
Which applications is GPR124 Polyclonal Antibody (bs-13515R) validated for, and at what dilution?
bs-13515R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPR124 Polyclonal Antibody (bs-13515R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse.
How should bs-13515R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPR124 Polyclonal Antibody (bs-13515R)?
KLH conjugated synthetic peptide derived from human G protein coupled receptor 124 Immunogen range: 351-450/1338.