GPR124 Polyclonal Antibody

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Rat Large intestine tissue lysates; Lane 4: Rat Lung tissue lysates ; Lane 5: U2os cell lysates probed with GPR124 Polyclonal Antibody, Unconjugated (bs-13515R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-13515R
Product Name	GPR124 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human G protein coupled receptor 124
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	25960
Swiss Prot	Q96PE1
Subcellular location	Cell membrane
Synonyms	DKFZp434C211; DKFZp434J0911; FLJ14390; G protein coupled receptor 124; G-protein coupled receptor 124; GP124_HUMAN; GPR 124; GPR124; KIAA1531; TEM 5; TEM5; Tumor endothelial marker 5; Tumor endothelial marker 5 precursor; GPCR GPR124; Adhesion G protein-coupled receptor A2; ADGRA2.
Background	Tumor endothelial markers (TEMs) are abundantly expressed in the blood vessels of human solid tumors during angiogenesis and neoangiogensis. These include TEM1 (endosialin), TEM5 (G-protein coupled receptor 124) and TEM7 (plexin domain containing 1). TEMs are associated with the cell surface membrane at low levels in normal human and mouse tissues. TEM5 is a seven-pass transmembrane receptor, whereas TEM1, TEM7 and TEM8 span the membrane once. TEM5 expression is elevated during tumor angiogenesis and neoangiogenesis. TEM7 is highly expressed in tumor endothelium and neurons. Therefore, TEM5 and TEM7 may be suitable targets for the development of antiangiogenic therapies.
Application Dilution
WB	ELISA1:500-1000
ELISA	IHC-P1:200-400
IHC-P	IHC-F1:100-500
IHC-F	IFIHC-P1:50-200
IF	IHC-F1:50-200
ICC/IF	IFICC1:50-200