Granzyme B Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1351R-BF647 |
| Product Name | Granzyme B Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Specific towards Granzyme B and Granzyme H |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GZMB |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3002 |
| Swiss Prot | P10144 |
| Subcellular location | Cytoplasm |
| Synonyms | HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; Granzyme B; C11; CTLA-1; Cathepsin G-like 1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; T-cell serine protease 1-3E; GZMB; GRB, Granzyme B, Granzyme H |
| Background | This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Granzyme B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1351R-BF647) validated for, and at what dilution?
bs-1351R-BF647 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Granzyme B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1351R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig.
How should bs-1351R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Granzyme B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1351R-BF647)?
KLH conjugated synthetic peptide derived from human GZMB
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-1351R-BF647)?
AbBy Fluor-647 on bs-1351R-BF647: excitation / emission 650nm/665nm.