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GPR4 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-13522r-fitc
Product Name GPR4 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human G protein coupled receptor 4
Immunogen Range 201-300/362
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2828
Subcellular location Cell membrane
Synonyms G protein coupled receptor 4; G-protein coupled receptor 4; G-protein coupled receptor 4; GPR 4; GPR4; GPR4_HUMAN; Probable G protein coupled receptor GPR4.
Background Enables G protein-coupled receptor activity. Involved in several processes, including G protein-coupled receptor signaling pathway; positive regulation of Rho protein signal transduction; and response to acidic pH. Located in plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GPR4 Polyclonal Antibody, FITC Conjugated (bs-13522R-FITC) validated for, and at what dilution?

bs-13522R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GPR4 Polyclonal Antibody, FITC Conjugated (bs-13522R-FITC) react with?

Tested reactivity: Human, Mouse.

How should bs-13522R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GPR4 Polyclonal Antibody, FITC Conjugated (bs-13522R-FITC)?

KLH conjugated synthetic peptide derived from human G protein coupled receptor 4 Immunogen range: 201-300/362.

What are the excitation and emission wavelengths of the FITC conjugate (bs-13522R-FITC)?

FITC on bs-13522R-FITC: excitation / emission 494nm/518nm.