GPR21 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13524R-Cy3 |
| Product Name | GPR21 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Cow, Pig, Horse, Monkey |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human G protein coupled receptor 21 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2844 |
| Subcellular location | Cell membrane |
| Synonyms | G protein coupled receptor 21; G protein coupled receptor gpr21; Gpr 21; Gpr21; Probable G protein coupled receptor 21; GPCR GPR21. |
| Background | G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR21 is a 349 amino acid multi-pass membrane protein that functions as an orphan receptor and belongs to the GPR1 family. The gene encoding GPR21 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome. Hereditary hemorrhagic telangiectasia, which is characterized by harmful vascular defects, and Familial dysautonomia, are both associated with chromosome 9. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GPR21 Polyclonal Antibody, Cy3 Conjugated (bs-13524R-Cy3) validated for, and at what dilution?
bs-13524R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPR21 Polyclonal Antibody, Cy3 Conjugated (bs-13524R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Monkey.
How should bs-13524R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPR21 Polyclonal Antibody, Cy3 Conjugated (bs-13524R-Cy3)?
KLH conjugated synthetic peptide derived from human G protein coupled receptor 21
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13524R-Cy3)?
Cy3 on bs-13524R-Cy3: excitation / emission 512,550nm/570,615nm.