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Beclin 1 Polyclonal Antibody

Lane 1: mouse brain lysates Lane 2: mouse intestine lysates probed with Anti BECN1\/Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 50kD.
Lane 1: mouse brain lysates Lane 2: mouse intestine lysates probed with Anti BECN1\/Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 50kD.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat stomach labeled with Anti-Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated(bs-1353R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin-embedded rat stomach labeled with Anti-Beclin 1\/ATG6 Polyclonal Antibody, Unconjugated(bs-1353R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beclin 1 Polyclonal Antibody (bs-1353R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beclin 1 Polyclonal Antibody (bs-1353R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. SMMC7721 cells probed with Rabbit Anti-Beclin 1 Polyclonal Antibody (bs-1353R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. SMMC7721 cells probed with Rabbit Anti-Beclin 1 Polyclonal Antibody (bs-1353R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:400 overnight at 4\u00b0C, DAB staining.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (bs-1353R ) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (bs-1353R ) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-1353R
Product Name Beclin 1 Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Beclin 1
Immunogen Range 201-330/450
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 8678
Swiss Prot Q14457
Subcellular location Cytoplasm, Cell membrane
Synonyms ATG6; VPS3; beclin1; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197
Background Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
FCM 1:100
IHC-P 1:100-500
IHC-F 1:100-500
IF(IHC-P) 1:100-500
IF(IHC-F) 1:100-500
IF(ICC) 1:100
ICC 1:100

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