ZBTB3 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13570R-BF680 |
| Product Name | ZBTB3 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZBTB3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | Zbtb3; ZBTB3_HUMAN; Zinc finger and BTB domain containing protein 3; Zinc finger and BTB domain-containing protein 3. |
| Background | The BTB is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. ZBTB3 (zinc finger and BTB domain containing 3) is a 574 amino acid protein that contains one BTB (POZ) domain and two C2H2-type zinc fingers. Localized to the nucleus, ZBTB3 is thought to play a role in transcriptional regulation events. The gene encoding ZBTB3 maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZBTB3 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13570R-BF680) validated for, and at what dilution?
bs-13570R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13570R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZBTB3 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13570R-BF680)?
KLH conjugated synthetic peptide derived from human ZBTB3
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-13570R-BF680)?
AbBy Fluor-680 on bs-13570R-BF680: excitation / emission 679nm/702nm.